Frame-projected independent fiber photometry (FIP) is a method to measure fluorescent sensor signals through optical fibers implanted in living animals, using a camera to record video of the fiber faces. To precisely control timings of excitation and camera frame acquisition for FIP, and of other external stimulation apparatus (e.g. LEDs for photo-stimulation), we use a Teensy 4.1 microcontroller, which generates voltage pulses without relying on continuous communication with the operating system of the experimental computer for timing. This protocol is for the 3 excitation LED + 2 collection CMOS cameras design.
The Surgery team offers a variety of aseptic rodent surgical procedures ranging from stereotaxic injections to headpost implantation and cranial windowing.
The Neuropixels platform uses pioneering technology for highly reproducible, targeted, brain-wide, cell-type-specific electrophysiology to record neural activity from defined neuron types across the brain.
The Molecular Anatomy platform combines innovative histology, imaging, and analysis techniques to map the morphology and molecular identity of neuron types across the whole brain.
The Behavior platform uses advanced technology to implement a standardized, modular, multi-task virtual reality gymnasium for mice, with the goal to study brain function across different behaviors at scale.
The Fiber Photometry platform enables optical measurement of neural activity in live animals to study neural circuits' function and dynamics in behaving animals.