Open Tool

Whole Brain Embedding for SmartSPIM - EasyIndex with 2% Agarose

Description

This modified LifeCanvas embedding protocol is intended to prepare cleared mouse brains for imaging on the SmartSPIM. It details mixing and degassing the agarose solution and the technical process of embedding a brain in the agarose solution.

Projects utilizing this tool

No items found.

Platforms utilizing this tool

No items found.

Platforms accelerate our work

Surgery

The Surgery team offers a variety of aseptic rodent surgical procedures ranging from stereotaxic injections to headpost implantation and cranial windowing.

Neuropixels Electrophysiology

The Neuropixels platform uses pioneering technology for highly reproducible, targeted, brain-wide, cell-type-specific electrophysiology to record neural activity from defined neuron types across the brain.

Brain-Wide Anatomy at Cellular Resolution

The Molecular Anatomy platform combines innovative histology, imaging, and analysis techniques to map the morphology and molecular identity of neuron types across the whole brain.

Fiber Photometry

The Fiber Photometry platform enables optical measurement of neural activity in live animals to study neural circuits' function and dynamics in behaving animals.

Behavior

The Behavior platform uses advanced technology to implement a standardized, modular, multi-task virtual reality gymnasium for mice, with the goal to study brain function across different behaviors at scale.